†Cardiovascular Well being Analysis Heart, Sanford Well being/College of South Dakota, Sioux Falls, SD USA
Abstract
Introduction
We now describe the outcomes of a follow-up research to look at the consequences of P-OM3, alone and together with aspirin, on collagen-mediated platelet signaling so as to determine mechanisms by which EPA and DHA impression platelet perform. Certainly, our earlier findings with entire blood aggregometry recommend that collagen-mediated platelet signaling could possibly be inhibited by 4 weeks of therapy with prescription omega-3 acid ethyl esters (P-OM3, Lovaza, GlaxoSmithKline) Collagen-mediated platelet signaling relies on the raft-bound GPVI receptor [18, 19], and due to this fact this pathway is a possible goal for modulation by DHA and EPA.
Supplies and Strategies
Outcomes
When the density of the bands at ~50 kDa and ~115 kDa (probably the most constantly noticed bands in all topics) had been quantified and represented by impact dimension, a big discount in band depth after collagen stimulation in comparison with baseline (Day 1) was noticed on Day 29 (Determine 4B, p=0.023), suggesting that intracellular signaling from collagen receptors is diminished after P-OM3 therapy. We then blotted for a phosphorylated model of the key collagen pathway signaling protein, PLCγ2, and located that ranges of collagen-stimulated PLCγ2 phosphorylation had been additionally diminished after EPA and DHA therapy (Fig. 4C) As seen in Determine 4A, most topics confirmed a discount in total collagen-mediated tyrosine phosphorylation after P-OM3 therapy when in comparison with baseline stimulation.
Dialogue and Conclusions
This research offers additional proof that platelet perform may be favorably affected by DHA and EPA with out adversely affecting regular hematology, and it means that no less than a part of the mechanism by which omega-3 fatty acids cut back threat for cardiovascular occasions is by way of results on platelet metabolism. In conclusion, an rising physique of proof continues to recommend EPA and DHA as promising therapeutics in combating heart problems. With their elevated use, it’ll change into crucial to raised perceive how these molecules affect mobile conduct.