How long does it take for biotin to get out your system? Biotin’s half-life is approximately 2 hours, so abstinence for 8 hours may be sufficient to minimize interference. However, it is highly recommended that patients taking massive biotin doses abstain for 72 hours before having blood specimens drawn for laboratory tests.
Does biotin build up in your system? Biotin is a water-soluble vitamin. That means it’s not stored in your body for long. Your body doesn’t naturally produce it, either.