Does biotin powder dissolve in water? I’ve known that biotin dissolves weakly in water, especially in low pH. In the PTM1 recipe, the biotin is 200 mg/L. This amount is the same with maximum solubility in water.
How do you dissolve D biotin in water? The issue with D-biotin is that it is a carboxylic acid and as such it decreases pH of aqueous solutions. Under acidic conditions D-biotin is however less soluble. My advice therefore is to use DMSO or an alkaline buffer (pH ~7.5) instead of water to prepare concentrated D-biotin solution.
Is biotin powder oil soluble? Biotin, also known as vitamin H or B7, is a water-soluble vitamin that helps the body metabolize fats, carbohydrates, and protein.
How To Make Biotin Solution
Working solution: Prepare 6 mL of an 80 µM working solution of biotin by diluting the 1 mM stock of biotin. Add 480 µL of 1 mM biotin to-52 mL of PBS and vortex solution to ensure mixing. Working solutions should be freshly prepared for each assay (within 24 hours). streptavidin instead of 5 mg.