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How To Make Biotin Solution?

Does biotin powder dissolve in water? I’ve known that biotin dissolves weakly in water, especially in low pH. In the PTM1 recipe, the biotin is 200 mg/L. This amount is the same with maximum solubility in water.

How do you dissolve D biotin in water? The issue with D-biotin is that it is a carboxylic acid and as such it decreases pH of aqueous solutions. Under acidic conditions D-biotin is however less soluble. My advice therefore is to use DMSO or an alkaline buffer (pH ~7.5) instead of water to prepare concentrated D-biotin solution.

Is biotin powder oil soluble? Biotin, also known as vitamin H or B7, is a water-soluble vitamin that helps the body metabolize fats, carbohydrates, and protein.

Related Questions

How To Make Biotin Solution

Working solution: Prepare 6 mL of an 80 µM working solution of biotin by diluting the 1 mM stock of biotin. Add 480 µL of 1 mM biotin to-52 mL of PBS and vortex solution to ensure mixing. Working solutions should be freshly prepared for each assay (within 24 hours). streptavidin instead of 5 mg.

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