How does biotin affect thyroid test results? Most commonly, biotin use can result in falsely high levels of T4 and T3 and falsely low levels of TSH, leading to either a wrong diagnosis of hyperthyroidism or that the thyroid hormone dose is too high.
Will 30 mcg of biotin affect thyroid tests? Biotin doses in standard multivitamin preparations (~30 mcg) are unlikely to impact lab tests. 3 However, supplements can have up to 650 times the recommended daily intake of biotin.
How much does biotin affect TSH levels? Two hours after ceasing 5 mg biotin, the levels of free T4, total T3, anti-TPO, anti-Tg, and anti-TSHR significantly increased (all P < . 001), while the level of TSH significantly decreased (P = . 005).