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FLIPR® Calcium Assay Kits are the platform of alternative for measuring adjustments of intracellular calcium for drug discovery and fundamental analysis.
FLIPR Calcium Assay Package
Constructed as probably the most complete portfolio of calcium reagents, the kits ship pre-optimized, homogeneous, fluorescence-based formulations to expedite assay growth and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits present probably the most complete methodology for detecting intracellular calcium adjustments in a easy and dependable homogeneous assay format. Choose from six formulations to enrich your goal of curiosity.
FLIPR Assay Kits make use of a quenching dye to cut back background fluorescence and enhance the signal-to-noise ratio. The patented quench expertise is obtainable to drug discovery and life science researchers solely by Molecular Gadgets, via the acquisition of FLIPR Assay Kits.
Key Assay Options and Advantages
Common mix-and-read protocol accelerates assay workflow and will increase throughput
Superior signal-to-noise ratio facilitates affirmation of endogenous or transiently transfected receptor exercise throughout assay growth
Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Techniques guarantee you may navigate each routine and unconventional cell strains and targets
Information of FLIPR Calcium Assay Kits
FLIPR Calcium 6 Assay Package sign window
Comparability of fluorescent sign response to calcium flux throughout carbachol agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios provided by homogenous assay run in media utilizing the FLIPR Calcium 6 Assay Package. Combining a novel fluorophore and the confirmed quench expertise, the FLIPR Calcium 6 Assay Package reveals the best sign to background ratio.
Quencher free sign window
When performing no-wash assays (preliminary media removing, changed with assay buffer, no-wash after dye-loading) the novel fluorophore of FLIPR Calcium 6 Assay Kits demonstrates highest sign to background ratio. Receive identical prime quality efficiency in presence or absence of masking dye.
Probenecid free sign window – “calcium 6 assay kit”
Novel fluorophore is extra immune to natural anion alternate proteins present in some cell strains. No sign seen when utilizing FLIPR Calcium 5 Assay Package with out probenecid (Ca5 Package no PBX). With FLIPR Calcium 6 Assay Package (Ca6), the sign is smaller with out probenecid, nevertheless EC50s are conserved and Z’ nonetheless larger than 0.5.
FLIPR Calcium 5, 4, & 3 Assay Package comparability
Comparability of fluorescent sign response to calcium flux throughout acetylcholine agonism of Muscarinic M1 receptor in CHO M1 cells. This illustrates the signal-to-noise ratios provided by numerous FLIPR Calcium Assay Kits with quench expertise. Combining the novel fluorophore and the confirmed quench expertise of the FLIPR Calcium 4 Assay Package, the FLIPR Calcium 5 Assay Package reveals increased sign to background ratio than prior kits.
Antagonism information with FLIPR Calcium 5 Assay Package
Antagonism of calcium flux in response to EC80 acetylcholine in CHO M1 cells evaluated with the FLIPR Calcium 5 Assay Package and the FLIPR Tetra System, demonstrating a big sign window and efficiency according to beforehand revealed information.