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Collagenase D

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Summary

1. Introduction

The current chapter describes the dissociation of human fetal skeletal muscle and the next purification of myoblasts utilizing cell sorting based mostly upon MCAM expression. In vitro tradition of those purified cells is then detailed in addition to an evaluation of myogenicity utilizing an in vitro fusion assay.

2. Supplies

3. Strategies

Acknowledgments

Footnotes

References

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