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For researchers involved about assay artifacts, the Fura-2 QBT™ Calcium Equipment is an easy, mix-and-read format that employs our proprietary masking know-how with the industry-standard Fura-2 ratiometric calcium indicator to precisely measure calcium mobilization.
Fura-2 QBT Calcium Equipment
The Fura-2 QBT Calcium Equipment eliminates the reason for knowledge variability and reduces the variety of steps in comparison with standard wash protocols utilizing Fura-2. Leveraging our proprietary masking know-how with the {industry} commonplace Fura-2 ratiometric calcium indicator, researchers can:
See largest Fura-2 sign window obtainable
Get rid of wash artifacts and enhance throughput with homogeneous assay
Reduce the influence of uneven dye loading and leakage on outcomes
Interrogate low-density, weakly or non-adherent cells utilizing no-wash protocol
Assay on FlexStation® 3 and FLIPR® Tetra Methods
Reference 1000’s of citations the place Fura-2 has been used for cell traces and goal
Knowledge of Fura-2 QBT Calcium Equipment
Agonism of the Muscarinic M1 Receptor on CHO M1 Cells
On the FlexStation® 3 Multi-Mode Microplate Reader, carbachol was used to stimulate the muscarinic M1 receptor on CHO M1 cells to generate focus response curves (CRC), evaluating Fura-2 QBT Dye to the BD Equipment and the normal Fura-2 wash protocol. EC50 values have been comparable for all strategies, and have been inside vary of printed values.
Antagonism of the Muscarinic M1 Receptor on CHO M1 Cells
On the FlexStation 3 Multi-Mode Microplate Reader, 50 nM carbachol was used as agonist problem versus an antagonist CRC of atropine. This experiment Fura-2 QBT Calcium Equipment supplied the most important sign window and most sturdy Z-factors at EC80.
Agonism of Endogenous H1 Receptor on HeLa Cells – “fura 2 calcium imaging”
Histamine was used to stimulate the endogenous histamine receptor on HeLa cells. Assay was measured utilizing the FLIPR Tetra® Excessive-Throughput Mobile Screening System with UV LEDs. Generated focus response curves (CRC) evaluating Fura-2 QBT Dye to the BD Ratiometric Calcium Equipment and conventional Fura-2 wash protocol. EC50 values have been inside a half log whereas the Z-factor at EC80 was the most important with the Fura-2 QBT Calcium Equipment.
Antagonism of Endogenous H1 Receptor on HeLa Cells
Assay was measured utilizing the FLIPR Tetra Excessive-Throughput Mobile Screening System with UV LEDs and used comparable experimental setup and system settings in Determine 3. 40 nM histamine was used as agonist problem versus an antagonist CRC of pyrilamine.
Know-how of Fura-2 QBT Calcium Equipment