†Cardiovascular Well being Analysis Heart, Sanford Well being/College of South Dakota, Sioux Falls, SD USA
(*3*)Abstract
(*3*)Introduction
We now describe the outcomes of a follow-up examine to look at the consequences of P-OM3, alone and together with aspirin, on collagen-mediated platelet signaling with a view to establish mechanisms by which EPA and DHA affect platelet operate. Certainly, our earlier findings with complete blood aggregometry counsel that collagen-mediated platelet signaling might be inhibited by 4 weeks of remedy with prescription omega-3 acid ethyl esters (P-OM3, Lovaza, GlaxoSmithKline) Collagen-mediated platelet signaling depends on the raft-bound GPVI receptor [18, 19], and due to this fact this pathway is a possible goal for modulation by DHA and EPA.
(*3*)Supplies and Strategies
(*3*)Outcomes
When the density of the bands at ~50 kDa and ~115 kDa (probably the most persistently noticed bands in all topics) have been quantified and represented by impact dimension, a big discount in band depth after collagen stimulation in comparison with baseline (Day 1) was noticed on Day 29 (Determine 4B, p=0.023), suggesting that intracellular signaling from collagen receptors is diminished after P-OM3 remedy. We then blotted for a phosphorylated model of the foremost collagen pathway signaling protein, PLCγ2, and located that ranges of collagen-stimulated PLCγ2 phosphorylation have been additionally diminished after EPA and DHA remedy (Fig. 4C) As seen in Determine 4A, most topics confirmed a discount in total collagen-mediated tyrosine phosphorylation after P-OM3 remedy when in comparison with baseline stimulation.
(*3*)Dialogue and Conclusions
This examine provides additional proof that platelet operate may be favorably affected by DHA and EPA with out adversely affecting regular hematology, and it means that at the very least a part of the mechanism by which omega-3 fatty acids scale back danger for cardiovascular occasions is by way of results on platelet metabolism. In conclusion, an growing physique of proof continues to counsel EPA and DHA as promising therapeutics in combating heart problems. With their elevated use, it’ll change into crucial to raised perceive how these molecules affect mobile conduct.
(*3*)Acknowledgements
(*3*)Footnotes