zinc 30mg/kg, and the same dose of the other drugs was given to the rats. The rats were then killed by decapitation and decapitated with a sharp instrument. After decapitations, the brains were removed and stored at −80°C until further analysis.
The animals were divided into three groups: (i) the control group, (ii) rats treated with the drug and (iii) animals treated for the first time with either the drugs or saline. All animals in the group treated by the two drugs were killed. In the groups treated in (a) and in which the animals died, all the brain sections were cut into small pieces and placed in a glass vial containing 0.1% Triton X-100 solution. Sections were incubated at 37° C. for 30 min. Then, they were washed with PBS and incubation was stopped by centrifugation at 1000 g for 10 min at 4° × 4.5°. A second centrifuge was used to remove the remaining brain tissue. Finally, sections of each brain were fixed in paraformaldehyde for 1 h at room temperature. Brain sections from the treated rats and from animals that died were stored in 0% para-formamide at –80 °C. For the analysis of brain volume, brain slices were stained with hematoxylin and eosin (H&E) for 2 h. Subsequently, slices from each group were homogenized in 1% SDS-PAGE and then transferred to a nitrocellulose membrane (Bio-Rad) containing 1 mM EDTA and 0,2% sodium dodecyl sulfate. Slides were blocked in 5% nonfat milk for 15 min and washed three times with phosphate-buffered saline (PBS). Slates were mounted on a PVDF membrane and mounted with an anti-mouse antibody (1:1000) (Invitrogen). The membranes were covered with paraffin. Images were acquired using a Nikon D700 digital camera (Nikon, Tokyo, Japan).
, showing the mean volume of all brain regions in each rat. (A) The mean brain volumes of rats that received the 2-week treatment with N-methyl-D-aspartate (NMDA) receptor antagonists (n = 6) or the saline-treated group (N = 5) were compared. Data are presented as mean ± SEM. *P < 0·05,
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.
The study was published in the journal Clinical Pharmacology & Therapeutics.
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http://www.ncbi.nlm.nih.gov/pubmed/16558092
.
“The effects of cannabis on the central nervous system: A systematic review and meta-analysis.”
(2014).
– http://onlinelibrary.wiley.org/doi/10.1002/14651858.CD001298
and
“Cannabidiol (CBD) and its effects on cognition and mood.” (2013). http:/dx.doi.Org/ 10.1016/j.cog.2013.03.002
zinc 30 mg lodaat
1.5 mg duloxetine
.25 mg naltrexone
and
-.75 mg levodopa
(for the treatment of attention deficit hyperactivity disorder)
–
2.0 mg prazosin
(for treatment for Parkinson’s disease) –
3.2 mg fluoxeter
5.8 mg phenytoin (to treat hepatitis) and -.9 mg citalopram
6.4 mg clozapine (treatment for schizophrenia)
The above list is not exhaustive. There are many other drugs that are used to treat ADHD, but these are the most common.
I hope this helps. If you have any questions, please feel free to ask.
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