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Summary
Alizarin purple S (ARS) is used for histological characterization of calcium. In an effort to enhance the situations of using the dye on organic supplies, the chemical response between ARS and Ca2+ is studied in vitro. In aqueous answer ARS and Ca2+ ions precipitate to kind brick-red deposits. For precipitation to happen a impartial pH (4 < pH < 8) is required; the stoichiometric ratio is 1:1. ARS reacts with calcium through its sulfonate and hydroxyl teams. The obvious solubility product is round 10∼7 Na+ and Okay+ ions don't intrude with equilibrium of the precipitation response however Mg2+ and soluble proteins comparable to bovine serum albumin react with ARS and kind soluble complexes which might result in a lower within the ARS amount out there to react with the calcium ions.
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