Affiliations
Analysis Unit of Medical Imaging, Physics and Know-how, College of Drugs, College of Oulu, Oulu, Finland,
Infotech Oulu, College of Oulu, Oulu, Finland,
Division of Diagnostic Radiology, Oulu College Hospital, Oulu, Finland
Figures
Summary
Introduction
The goal of this examine was to make clear whether or not conventional collagen stains, resembling Masson’s trichrome or Picrosirius purple, are appropriate for the quantification of the collagen content material in histological sections of AC. As well as, polarized gentle microscopy (PLM) was used to measure the retardance of unstained sections and Picrosirius red-stained sections. In precept, DD is also utilized to histological sections stained with collagen particular dyes.
Supplies and strategies
Outcomes
In case of Picrosirius purple, the elimination of PGs earlier than staining (Fig 1D) considerably improved the staining in comparison with the Picrosirius purple protocol wherein PGs weren’t eliminated earlier than staining (Fig 1C). Masson (Fig 1A) and Modified Masson (Fig 1B) look related, count on for the shortage of purple coloration in Modified Masson because the Biebrich scarlet-acid fuchsin was not used. Examples of a cartilage pattern stained utilizing the totally different collagen staining protocols are proven in Fig 1.
Dialogue and conclusions
Utilizing histological slides as a substitute of enormous tissue items for the quantification decreases the required variety of samples to acquire statistically important outcomes and permits the comparability of a similar pattern with numerous methods requiring histological sections resembling FTIR spectroscopic imaging, PLM, histology, and immunohistochemistry. In conclusion, this new quantitative histological technique for collagen staining permits spatial quantification of the collagen distribution in the entire part which is tough and even not possible by commonplace biochemical evaluation strategies[3–5]. The histological quantification additionally opens new views for much less harmful collagen quantification, in comparison with beforehand used HPLC and hydroxyproline biochemical evaluation[3–5].
Acknowledgments
We thank Ms. Tarja Huhta for technical help.