Vijaya Vegesna, James O’Kelly, Milan Uskokovic, Jonathan Mentioned, Nathan Lemp, Takayuki Saitoh, Takayuki Ikezoe, Lise Binderup, H. Phillip Koeffler, Vitamin D3 Analogs Stimulate Hair Progress in Nude Mice, Endocrinology, Quantity 143, Situation 11, 1 November 2002, Pages 4389–4396, https://doi.org/10.1210/en.2002-220118
Summary
These observations inspired us to discover the flexibility of vitamin D3 analogs to stimulate hair progress in nude mice with defects in keratinocytes. Numerous vitamin D3 analogs have been developed which might be stronger of their potential to induce differentiation and inhibit proliferation however produce much less hypercalcemia than the physiologically lively compound, 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] (9). We selected six vitamin D3 analogs that matched these traits and examined their potential to trigger hair progress in nude mice (10–15).
Supplies and Strategies
Antisera towards keratin proteins had been used for Western blotting to determine expression patterns on the protein stage (16, 17). One antisera was towards kind I acidic hair keratin Ha8, which usually exhibits complicated a expression sample in rising follicles; and the second acknowledged kind II primary hair keratin Hb3.
Outcomes
To handle whether or not the impact of hair progress stimulation by vitamin D3 analogs is restricted to nude mice, C57 BL/6 mice got both Ro 25-9022 or Ro 26-9114, 3 times per week, after hair regrowth was stimulated by plucking of the hair. Hair scores had been taken 3 times per week utilizing the identical scale as described in Desk 1. No acceleration within the regrowth of hair occurred throughout remedy with both of the vitamin D3 analogs (Fig. 7).
Dialogue
In our experiments, the administration of 1,25(OH)2D3 didn’t induce hair progress within the nude mice; nonetheless, quite a lot of vitamin D3 analogs did trigger hair progress. All of those analogs have been beforehand proven to have a higher efficiency to induce differentiation and inhibit proliferation of quite a lot of cancer-related cell sorts, each in vitro and in vivo (10–15) (Desk 3). Due to this fact, the doubtless purpose why the vitamin D3 analogs are in a position to stimulate hair progress in nude mice, whereas 1,25(OH)2D3 can’t, is that they’ve higher efficiency in vivo. Every of the analogs is ready to bind to VDR (9). A few of the mechanisms which might be hypothesized to account for his or her elevated efficiency embody the truth that they’ve decreased binding to D-binding protein within the serum, which permits extra prepared entry to getting into the cell. These analogs might alter the conformation of the VDR, making them extra lively, or they could lengthen the half-life of the activated VDR (34). They may be modulating the flexibility of corepressors or coactivators to work together with the ligand-activated VDR (35). Regardless of the mechanism could also be, these analogs are appearing on the hair follicle of the nude mice, in all probability by stimulating the expression of genes, akin to keratins, that are downstream of the faulty Whn transcription issue; and these genes are vital to formation of the hair shaft (16, 17, 31). In abstract, our outcomes present sturdy proof that vitamin D3 analogs can stimulate the expansion of hair in nude mice that usually wouldn’t have seen hair.
Acknowledgments – “does vitamin d grow hair”
This work was supported, partially, by the Sid and Ann Schwartz household fund, the Horn Belief, and the Parker Hughes Basis. H.P.Ok. is the holder of the Mark Goodson Chair in Oncology at Cedars Sinai Medical Heart and is a member of the Jonsson Most cancers Heart.
Abbreviations
“does vitamin d grow hair”